A52 MERS coronaviruses from camels in Africa exhibit region-dependent genetic diversity
Identifieur interne : 000718 ( Main/Exploration ); précédent : 000717; suivant : 000719A52 MERS coronaviruses from camels in Africa exhibit region-dependent genetic diversity
Auteurs : D K W. Chu ; K P Y. Hui ; R A P M. Perera ; E. Miguel [France] ; J O Oladipo [Nigeria] ; A. Traore ; O. Fassi-Fihri [Maroc] ; M C W. Chan ; Z. Zhou ; R T Y. So ; V. Chevalier [France] ; J S M. PeirisSource :
- Virus Evolution [ 2057-1577 ] ; 2019.
Abstract
Middle East respiratory syndrome coronavirus (MERS-CoV) causes a zoonotic respiratory disease of global public health concern, and dromedary camels are the only proven source of this zoonotic infection. Although MERS-CoV infection is ubiquitous in dromedaries across Africa and the Arabian Peninsula, the continuous appearance of zoonotic MERS cases in humans is confined to the Arabian Peninsula. MERS-CoV from Africa has hitherto been poorly studied. Here, we report the genetic and phenotypic characterization of MERS-CoV from dromedaries in African countries. Phylogenetically, viruses from dromedaries in Africa formed a monophyletic clade, which we have provisionally designated as virus clade C. Molecular dating analyses of MERS-CoV, including clade C viruses, suggests that the ancestral MERS-CoV in dromedaries could have spread to the two continents within a short timeframe. Camel MERS-CoVs from west and north African countries form a subclade (C1) that shares genetic signatures of a major deletion in the accessory gene ORF4b. Compared with human and camel MERS-CoV from Saudi Arabia, virus isolates from Burkina Faso (BF785) and Nigeria (Nig1657) had lower virus replication competence in Calu-3 cells and in
Url:
DOI: 10.1093/ve/vez002.051
PubMed: NONE
PubMed Central: 6735769
Affiliations:
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Chan</name><affiliation><nlm:aff id="vez002.051-AFF1">School of Public Health, Li Ka Shing Faculty of Medicine, The University of Hong Kong</nlm:aff><wicri:noCountry code="subfield">The University of Hong Kong</wicri:noCountry></affiliation></author><author><name sortKey="Zhou, Z" sort="Zhou, Z" uniqKey="Zhou Z" first="Z" last="Zhou">Z. Zhou</name><affiliation><nlm:aff id="vez002.051-AFF1">School of Public Health, Li Ka Shing Faculty of Medicine, The University of Hong Kong</nlm:aff><wicri:noCountry code="subfield">The University of Hong Kong</wicri:noCountry></affiliation></author><author><name sortKey="So, R T Y" sort="So, R T Y" uniqKey="So R" first="R T Y" last="So">R T Y. So</name><affiliation><nlm:aff id="vez002.051-AFF1">School of Public Health, Li Ka Shing Faculty of Medicine, The University of Hong Kong</nlm:aff><wicri:noCountry code="subfield">The University of Hong Kong</wicri:noCountry></affiliation></author><author><name sortKey="Chevalier, V" sort="Chevalier, V" uniqKey="Chevalier V" first="V" last="Chevalier">V. Chevalier</name><affiliation wicri:level="1"><nlm:aff id="vez002.051-AFF2">Animal, Santé, Territoires, Risques et Ecosystèmes, Centre de Coopération Internationale en Recherche Agronomique pour le Développement, Institut National de la Recherche Agronomique, Université de Montpellier, France</nlm:aff><country xml:lang="fr">France</country><wicri:regionArea>Animal, Santé, Territoires, Risques et Ecosystèmes, Centre de Coopération Internationale en Recherche Agronomique pour le Développement, Institut National de la Recherche Agronomique, Université de Montpellier</wicri:regionArea><wicri:noRegion>Université de Montpellier</wicri:noRegion><wicri:noRegion>Université de Montpellier</wicri:noRegion></affiliation></author><author><name sortKey="Peiris, J S M" sort="Peiris, J S M" uniqKey="Peiris J" first="J S M" last="Peiris">J S M. Peiris</name><affiliation><nlm:aff id="vez002.051-AFF1">School of Public Health, Li Ka Shing Faculty of Medicine, The University of Hong Kong</nlm:aff><wicri:noCountry code="subfield">The University of Hong Kong</wicri:noCountry></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmc">6735769</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6735769</idno><idno type="RBID">PMC:6735769</idno><idno type="doi">10.1093/ve/vez002.051</idno><idno type="pmid">NONE</idno><date when="2019">2019</date><idno type="wicri:Area/Pmc/Corpus">000641</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000641</idno><idno type="wicri:Area/Pmc/Curation">000641</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000641</idno><idno type="wicri:Area/Pmc/Checkpoint">000414</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">000414</idno><idno type="wicri:Area/Ncbi/Merge">002B72</idno><idno type="wicri:Area/Ncbi/Curation">002B72</idno><idno type="wicri:Area/Ncbi/Checkpoint">002B72</idno><idno type="wicri:Area/Main/Merge">000721</idno><idno type="wicri:Area/Main/Curation">000718</idno><idno type="wicri:Area/Main/Exploration">000718</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">A52 MERS coronaviruses from camels in Africa exhibit region-dependent genetic diversity</title><author><name sortKey="Chu, D K W" sort="Chu, D K W" uniqKey="Chu D" first="D K W" last="Chu">D K W. Chu</name><affiliation><nlm:aff id="vez002.051-AFF1">School of Public Health, Li Ka Shing Faculty of Medicine, The University of Hong Kong</nlm:aff><wicri:noCountry code="subfield">The University of Hong Kong</wicri:noCountry></affiliation></author><author><name sortKey="Hui, K P Y" sort="Hui, K P Y" uniqKey="Hui K" first="K P Y" last="Hui">K P Y. Hui</name><affiliation><nlm:aff id="vez002.051-AFF1">School of Public Health, Li Ka Shing Faculty of Medicine, The University of Hong Kong</nlm:aff><wicri:noCountry code="subfield">The University of Hong Kong</wicri:noCountry></affiliation></author><author><name sortKey="Perera, R A P M" sort="Perera, R A P M" uniqKey="Perera R" first="R A P M" last="Perera">R A P M. Perera</name><affiliation><nlm:aff id="vez002.051-AFF1">School of Public Health, Li Ka Shing Faculty of Medicine, The University of Hong Kong</nlm:aff><wicri:noCountry code="subfield">The University of Hong Kong</wicri:noCountry></affiliation></author><author><name sortKey="Miguel, E" sort="Miguel, E" uniqKey="Miguel E" first="E" last="Miguel">E. Miguel</name><affiliation wicri:level="1"><nlm:aff id="vez002.051-AFF2">Animal, Santé, Territoires, Risques et Ecosystèmes, Centre de Coopération Internationale en Recherche Agronomique pour le Développement, Institut National de la Recherche Agronomique, Université de Montpellier, France</nlm:aff><country xml:lang="fr">France</country><wicri:regionArea>Animal, Santé, Territoires, Risques et Ecosystèmes, Centre de Coopération Internationale en Recherche Agronomique pour le Développement, Institut National de la Recherche Agronomique, Université de Montpellier</wicri:regionArea><wicri:noRegion>Université de Montpellier</wicri:noRegion><wicri:noRegion>Université de Montpellier</wicri:noRegion></affiliation></author><author><name sortKey="Oladipo, J O" sort="Oladipo, J O" uniqKey="Oladipo J" first="J O" last="Oladipo">J O Oladipo</name><affiliation wicri:level="1"><nlm:aff id="vez002.051-AFF3">Department of Surgery, Faculty of Clinical Sciences, University of Ilorin, Nigeria</nlm:aff><country xml:lang="fr">Nigeria</country><wicri:regionArea>Department of Surgery, Faculty of Clinical Sciences, University of Ilorin</wicri:regionArea><wicri:noRegion>University of Ilorin</wicri:noRegion></affiliation></author><author><name sortKey="Traore, A" sort="Traore, A" uniqKey="Traore A" first="A" last="Traore">A. Traore</name><affiliation><nlm:aff id="vez002.051-AFF4">Laboratoire de Biologie et Santé Animals, L’Institut de l’Environnement et de Recherches Agricoles du Burkina Faso</nlm:aff><wicri:noCountry code="subfield">L’Institut de l’Environnement et de Recherches Agricoles du Burkina Faso</wicri:noCountry></affiliation></author><author><name sortKey="Fassi Fihri, O" sort="Fassi Fihri, O" uniqKey="Fassi Fihri O" first="O" last="Fassi-Fihri">O. Fassi-Fihri</name><affiliation wicri:level="1"><nlm:aff id="vez002.051-AFF5">Institut Agronomique et Vétérinaire, Hassan II Universite, Morocco</nlm:aff><country xml:lang="fr">Maroc</country><wicri:regionArea>Institut Agronomique et Vétérinaire, Hassan II Universite</wicri:regionArea><wicri:noRegion>Hassan II Universite</wicri:noRegion></affiliation></author><author><name sortKey="Chan, M C W" sort="Chan, M C W" uniqKey="Chan M" first="M C W" last="Chan">M C W. Chan</name><affiliation><nlm:aff id="vez002.051-AFF1">School of Public Health, Li Ka Shing Faculty of Medicine, The University of Hong Kong</nlm:aff><wicri:noCountry code="subfield">The University of Hong Kong</wicri:noCountry></affiliation></author><author><name sortKey="Zhou, Z" sort="Zhou, Z" uniqKey="Zhou Z" first="Z" last="Zhou">Z. Zhou</name><affiliation><nlm:aff id="vez002.051-AFF1">School of Public Health, Li Ka Shing Faculty of Medicine, The University of Hong Kong</nlm:aff><wicri:noCountry code="subfield">The University of Hong Kong</wicri:noCountry></affiliation></author><author><name sortKey="So, R T Y" sort="So, R T Y" uniqKey="So R" first="R T Y" last="So">R T Y. So</name><affiliation><nlm:aff id="vez002.051-AFF1">School of Public Health, Li Ka Shing Faculty of Medicine, The University of Hong Kong</nlm:aff><wicri:noCountry code="subfield">The University of Hong Kong</wicri:noCountry></affiliation></author><author><name sortKey="Chevalier, V" sort="Chevalier, V" uniqKey="Chevalier V" first="V" last="Chevalier">V. Chevalier</name><affiliation wicri:level="1"><nlm:aff id="vez002.051-AFF2">Animal, Santé, Territoires, Risques et Ecosystèmes, Centre de Coopération Internationale en Recherche Agronomique pour le Développement, Institut National de la Recherche Agronomique, Université de Montpellier, France</nlm:aff><country xml:lang="fr">France</country><wicri:regionArea>Animal, Santé, Territoires, Risques et Ecosystèmes, Centre de Coopération Internationale en Recherche Agronomique pour le Développement, Institut National de la Recherche Agronomique, Université de Montpellier</wicri:regionArea><wicri:noRegion>Université de Montpellier</wicri:noRegion><wicri:noRegion>Université de Montpellier</wicri:noRegion></affiliation></author><author><name sortKey="Peiris, J S M" sort="Peiris, J S M" uniqKey="Peiris J" first="J S M" last="Peiris">J S M. Peiris</name><affiliation><nlm:aff id="vez002.051-AFF1">School of Public Health, Li Ka Shing Faculty of Medicine, The University of Hong Kong</nlm:aff><wicri:noCountry code="subfield">The University of Hong Kong</wicri:noCountry></affiliation></author></analytic><series><title level="j">Virus Evolution</title><idno type="eISSN">2057-1577</idno><imprint><date when="2019">2019</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><title>Abstract</title><p>Middle East respiratory syndrome coronavirus (MERS-CoV) causes a zoonotic respiratory disease of global public health concern, and dromedary camels are the only proven source of this zoonotic infection. Although MERS-CoV infection is ubiquitous in dromedaries across Africa and the Arabian Peninsula, the continuous appearance of zoonotic MERS cases in humans is confined to the Arabian Peninsula. MERS-CoV from Africa has hitherto been poorly studied. Here, we report the genetic and phenotypic characterization of MERS-CoV from dromedaries in African countries. Phylogenetically, viruses from dromedaries in Africa formed a monophyletic clade, which we have provisionally designated as virus clade C. Molecular dating analyses of MERS-CoV, including clade C viruses, suggests that the ancestral MERS-CoV in dromedaries could have spread to the two continents within a short timeframe. Camel MERS-CoVs from west and north African countries form a subclade (C1) that shares genetic signatures of a major deletion in the accessory gene ORF4b. Compared with human and camel MERS-CoV from Saudi Arabia, virus isolates from Burkina Faso (BF785) and Nigeria (Nig1657) had lower virus replication competence in Calu-3 cells and in <italic>ex vivo</italic> cultures of human bronchus and lung, and BF785 replicated to lower titer in lungs of human DPP4-transduced mice. However, it is still inconclusive whether ORF4b deletions may lead to the reduced replication competence of BF785 and Nig1657. Genetic and phenotypic differences in West African viruses may be relevant to the zoonotic potential of MERS-CoV.</p></div></front></TEI><affiliations><list><country><li>France</li><li>Maroc</li><li>Nigeria</li></country></list><tree><noCountry><name sortKey="Chan, M C W" sort="Chan, M C W" uniqKey="Chan M" first="M C W" last="Chan">M C W. Chan</name><name sortKey="Chu, D K W" sort="Chu, D K W" uniqKey="Chu D" first="D K W" last="Chu">D K W. Chu</name><name sortKey="Hui, K P Y" sort="Hui, K P Y" uniqKey="Hui K" first="K P Y" last="Hui">K P Y. Hui</name><name sortKey="Peiris, J S M" sort="Peiris, J S M" uniqKey="Peiris J" first="J S M" last="Peiris">J S M. Peiris</name><name sortKey="Perera, R A P M" sort="Perera, R A P M" uniqKey="Perera R" first="R A P M" last="Perera">R A P M. Perera</name><name sortKey="So, R T Y" sort="So, R T Y" uniqKey="So R" first="R T Y" last="So">R T Y. So</name><name sortKey="Traore, A" sort="Traore, A" uniqKey="Traore A" first="A" last="Traore">A. Traore</name><name sortKey="Zhou, Z" sort="Zhou, Z" uniqKey="Zhou Z" first="Z" last="Zhou">Z. Zhou</name></noCountry><country name="France"><noRegion><name sortKey="Miguel, E" sort="Miguel, E" uniqKey="Miguel E" first="E" last="Miguel">E. Miguel</name></noRegion><name sortKey="Chevalier, V" sort="Chevalier, V" uniqKey="Chevalier V" first="V" last="Chevalier">V. Chevalier</name></country><country name="Nigeria"><noRegion><name sortKey="Oladipo, J O" sort="Oladipo, J O" uniqKey="Oladipo J" first="J O" last="Oladipo">J O Oladipo</name></noRegion></country><country name="Maroc"><noRegion><name sortKey="Fassi Fihri, O" sort="Fassi Fihri, O" uniqKey="Fassi Fihri O" first="O" last="Fassi-Fihri">O. 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